Microbiology Study

41.-50. Lab Questions. Matching. Match the correct letter and number. You may not need to use all the letters, and a letter may be used more than once; but pick only the one letter that best matches with the number.

A. high power objective

B. oil immersion objective

C. iris diaphragm

D. mechanical stage

E. parfocal

F. rod (bacillus) morphology

G. coccus morphology

H. basic stain

I. spiral morphology

J. safranin

K. resolution

L. heat fixation

M. Enterobacter aerogenes

N. sun screen

41.________controls the amount of light passing through the condenser.

42.________causes bacteria to adhere to the slide for staining

43.________Bacillus thuringiensis

44.________100 X

45.________ counterstain in the Gram stain

46.________inversely related to numerical aperture

47.________Lactococcus lactis

48.________focused with low power, then should be approximately in focus with oil immersion lens in place.

49. _______crystal violet

50._______Pseudomonas fluorescens

BIOL 205 Exam#1 Spring 2006

1.-11. Write positive if found only in a gram positive bacterial cell; write negative if found only in a gram negative bacterial cell; write positive/negative if found in both.

1.DNA_____________

2. RNA_____________

3. lipopolysaccharide____________________

4. phospholipids________________

5. porins_____________________

6. ribosomes______________________

7.lipoteichoic acid_____________________

8. peptidoglycan___________________

9. flagella_______________________

10. plasma membrane transport proteins_______________________

11. N-acetylglucosamine_____________________

12.-23. Place a P after a molecule or structure found in a prokaryote cell (domain: Bacteria) and an E after a molecule or structure found in a eukaryote cell (either P or E is the answer, NOT both).

12. 18S rRNA_____________

13. 80S ribosomes_____________

14. mitochondria_______________

15. N-acetylmuramic acid_______________

16. endospores________________

17. endotoxin______________

18. pili____________________

19. lysosomes________________

20. nuclear envelope_________________

21. Golgi bodies__________________

22. endoplasmic reticulum__________________

23.-32. True or False. Write out True or False.

23. Direct microscopic counts of a bacterial population would likely give a lower count

than a plate count.___________________

24. Pili (or fimbriae) function in attachment to surfaces.___________________

25. Eukaryote cytoplasmic ribosomes are often affected by antibiotics that inhibit bacterial protein synthesis.___________________

26. A bacterial generation time is usually calculated during the death phase of growth._____________________

27. Bacteria regulate gylcolysis by turning genes on and off.._______________

28. Glucose is the usual C-source for an autotroph _____________________

29. Anaerobic bacteria can not generate ATP.___________________

30. Capsular material usually consists of polysaccharide..___________________

31. The bacterial "chromosome" is usually a linear strand of DNA.___________________

32. "70S" ribosomes are found in bacteria, chloroplasts, and mitochondria.____________________

33.-40. Word List. A word may be used once, more than once, or not at all.

ATPase

Ca dipicolinate

Cell membrane

Chemotaxis

Cytoskeleton

Lysozyme

Magnetosome

Nucleoid

Penicillin

Peritrichous

Pili

Plasmid

Polar

Poly-B-OH-butyric acid

Precursor